Observation of a single-beam gradient force optical trap for dielectric particles. Monitoring the kinetics of Bacillus subtilis endospore germination via surface-enhanced Raman scattering spectroscopy. Monitoring DPA release from a single germinating Bacillus subtilis endospore via surface-enhanced Raman scattering microscopy. Flow cytometric assessment of Bacillus spore response to high pressure and heat. Mathys, A., Chapman, B., Bull, M., Heinz, V. A microtiter fluorometric assay to detect the germination of Bacillus anthracis spores and the germination inhibitory effects of antibodies. Factors influencing germination of Bacillus subtilis spores via activation of nutrient receptors by high pressure. Assessment of bacterial endospore viability with fluorescent dyes. Analysis of nucleoid morphology during germination and outgrowth of spores of Bacillus species. In vitro high-resolution structural dynamics of single germinating bacterial spores. Plomp, M., Leighton, T.J., Wheeler, K.E., Hill, H.D. Imaging and analysis of Bacillus anthracis spore germination. Dipicolinic acid (DPA) assay revisited and appraised for spore detection. Real time monitoring of Bacillus subtilis endospore components by attenuated total reflection Fourier transform infrared spectroscopy during germination. Study of calcium dipicolinate release during bacterial spore germination by using a new, sensitive assay for dipicolinate. Release of small molecules during germination of spores of Bacillus species. & Setlow, P.) 43–63 (American Society for Microbiology, 1989). In Regulation of Prokaryotic Development (eds. Spores of Bacillus subtilis: their resistance to radiation, heat and chemicals. Resistance of Bacillus endospores to extreme terrestrial and extraterrestrial environments. Nicholson, W.L., Munakata, N., Horneck, G., Melosh, H.J. In Food Microbiology: Fundamentals And Frontiers 3rd edn. The method can also be adapted to use multi-trap Raman spectroscopy or phase-contrast microscopy of spores adhered on a cover slip to simultaneously obtain germination parameters for multiple individual spores. This information leads to precise correlations between various germination events, and takes 1–2 h to complete. The characterization consists of the following steps: (i) loading heat-activated dormant spores into a temperature-controlled microscope sample holder containing a germinant solution plus a nucleic acid stain (ii) capturing a single spore with optical tweezers (iii) simultaneously measuring phase-contrast images, Raman spectra and fluorescence images of the optically captured spore at 2- to 10-s intervals and (iv) analyzing the acquired data for the loss of spore refractility, changes in spore-specific molecules (in particular, dipicolinic acid) and uptake of the nucleic acid stain. This protocol describes a method combining phase-contrast and fluorescence microscopy, Raman spectroscopy and optical tweezers to characterize the germination of single bacterial spores.
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KRS’ first hit, “The Bridge Is Over,” was a diss to the borough of Queens, somewhat of a second birthplace for New York rap. producers Show, Lord Finesse, Buckwild and Diamond D continue to provide backdrops for Bronx classics. A few years later, Joey Crack introduced the world to the legendary Big Pun. With KRS-One as its perennial rap mayor, this is the borough that brought us Fat Joe, who stepped in the scene in 1995 with limited skills and a whole lot of realness. New York is where it all started, South Bronx to be exact, and the BX still represents that true-school heritage. Cop an album like Nas’ 1994 classic Illmatic and you’ll get a real musical representation of the NY state of mind. Forget the fact that New Yorkers now like to get crunk and throw elbows like they were Tupac fans all along. To this day, East Coast MCs are measured against the meticulously constructed rhymes of legends like Rakim or Big Daddy Kane, while beatsmiths strive to attain DJ Premier-like perfection. 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Pray With Me: Help Your Children To Engage in Authentic and Powerful Prayer. I hope this episode is helpful for you as you are encouraging your child to pray and talk to God.Īnd grab a copy of Erica’s book for even more ideas on how you can teach your kids about prayer. If you’d like more ideas for bedtime routines, check out Episode 68 - Bedtime Routine Ideas For Families. What a great way to end the day! You can give gratitude back to God for the day, pray for people, the next day, or things coming up. Lighting the candle can signify that it’s prayer time and help children focus on the task at hand.Īs you’re going through your bedtime routine, end it with prayer. Spend however long you want (or can) in family prayer time. Clear the table, light a candle, and turn the lights off. Light a candle when praying at home (my favorite of the ideas!)įirst, pick a time to regularly pray together as a family - after dinner for example. This is a great way to teach your children how to pray for people. On your daily route, pick a house or building you see and pray for the people who live there, go there, shop there, or work there. Pick a building or house and pray for the people in it. Whatever is going to work best for your family. It can be the same thing every day or something different. Let the kids help you pick what you pray for, too. Everyone in the car can be on the lookout and shout out when they see it, then everyone can pray out loud together or silently. When you’re passing a certain bridge, you can say a quick prayer. When you’re out and about with your kids during your daily errands and activities, you can use the things you see every day as visual cues to pray. Pick a particular landmark to remind you to pray. Here are 4 ways that you can incorporate prayer into your everyday life with your kids. Praying to God is a privilege and something that we should be eager to teach our children. Modeling when to pray, how to pray, and what to pray for is key to teaching your child that praying to God is something really special. I'm so happy to have her with us on this episode sharing about this incredible topic! 5 Ways Families Can Pray Together My guest, Erica Renaud has just released an incredible book that goes deeper into teaching children how to pray and helping them engage in prayer with God. This episode is packed with simple ideas on ways you can model prayer in your day-to-day life and pray together as a family. When you think about how amazing it is that we have access to God through prayer, it's actually pretty mind-blowing!Īs you are pointing your child to Christ through conversations at home, Bible reading, and going to church, you can also teach them what it means to pray. Praying to God is a big part of the Christian life. You can read through this blog post and do these activities with your kids, or you can listen to this episode with your kids, play the games, and use the episode content as conversation starters as we move through the topics/ideas. Along For The Ride is an interactive game and talk show for kids and parents. |
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